Bryan Johnson anchored OS Fund with $100 million of his own capital to back founders building what he calls "tools of creation"—computer software, genomics, AI, robotics, and synthetic biology—that can "rewrite the operating systems of life" rather than just create applications. He filters entrepreneurs primarily on "future literacy," looking for those who understand how emerging technologies will reshape the world and build accordingly, while developing an open-source playbook for non-scientist investors to systematically evaluate hard science risks.

• A conviction that biological health drives professional output, viewing the grind mentality as a form of metabolic dysfunction. He noted that healthy individuals produce significantly more work than unhealthy ones, arguing that physical wellness is essential for writing good code and maintaining high efficiency.
• An intense focus on sleep optimization as a primary tool for productivity and moral behavior. He cited studies linking lack of sleep to dishonesty and shared his personal experiments with rocking devices to potentially improve memory consolidation.
• A reliance on rigorous data collection to quantify health and aging. He frequently discusses measuring specific biomarkers, such as core body temperature via inner ear versus rectal devices, to ensure his longevity protocols are scientifically accurate.
• A view of Artificial Intelligence as a fundamental shift in civilization, comparable to the printing press or fire. He contextualizes the rapid development of AI by comparing the countdown to graduation with the countdown to the technological singularity.
• Interest in the medical and biological effects of psychedelics. He detailed an experiment tracking hundreds of biomarkers to analyze how magic mushrooms influenced his microbiome and acted as a hard reset for his thought patterns.
• Advocacy for kindness and emotional regulation as indicators of peak biological performance. He described kindness as requiring metabolic abundance and executive function, whereas he categorized meanness as a symptom of low energy and inflammation.
• Periodic detachment from social media to regulate mental health. He organizes community fasts from platforms for 36 hours to combat anxiety and the brain's negativity bias, encouraging others to reset their nervous systems.